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Invitrogen on X: "Not sure what's the right annealing temperature for your #MolecularBiology experiment when using different DNA polymerases? Check out the Tm Calculator and get a quick estimate: https://t.co/rK9pwxOg8Z #Polymerase #LabTips #
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Tm Calculator
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SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
Versatility of different melting temperature (Tm) calculator software for robust PCR and real-time PCR oligonucleotide design: A practical guide | Mohammad Javad Najafpanah - Academia.edu
NEB Tm Calculator – LabTools
Tm calculation
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Annealing Temperature Calculator - Calculator Academy
Annealing Temperature Calculator | PCR Method
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